Diabetes Research Center (DRC)
Washington University in St. Louis

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Main page of DRC site, legends in text below.

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What's New

The DRC has, in collaboration with the newly formed Washington University Center for Cellular Imaging (WUCCI), established a pilot scholarship program to support use of the WUCCI by its members to bring advanced cellular imaging and analysis tools to basic and translational studies of diabetes. The pilot program will provide a single $500 scholarship to DRC member laboratories for use in covering the costs of pilot cellular imaging studies using the WUCCI. The DRC has established resources for a total of 40 scholarships, and these are available to DRC members on a first come, first served basis.

Recent advances in cellular imaging enable unprecedented dynamic and spatial resolution in studies of cells, tissues, and organisms, creating exciting opportunities for discovery in a broad range of research aimed at understanding the cellular mechanisms that underlie diabetes pathogenesis. The WUCCI provides access to cutting-edge microscopy and sample preparation equipment along with technical expertise to promote and facilitate the highest possible quality cellular imaging and analysis approaches. The WUCCI is located in the basement of the new 4515 McKinley Research Building.

The capabilities of the WUCCI are briefly summarized below:

Light Microscopy
Basic fluorescence microscopy / stereoscopy (widefield)
Confocal microscopy (both fixed and high-speed live cell on inverted and upright platforms)
TIRF microscopy (live cell) Two-photon microscopy (both fixed tissue and in vivo intravital)
Super-resolution microscopy (both localization and structured illumination)

Electron & Ion Microscopy
Transmission Electron Microscopy (TEM)
Scanning Electron Microscopy (SEM)
3D EM using Focused Ion Beam (FIB-SEM)
TEM sample prep including embedding, thin section and deep etch EM
SEM sample prep including sputter coating and block finishing for 3DEM

Other Facilities
Wet / chemical lab space for sample prep including sample freezing
Sectioning space for thin section production for TEM
BSL-2 tissue culture space including BSCs and incubators (both CO2 and tri-gas)
Animal holding closet for short-term (< 24hr imaging studies)

More detailed information on the WUCCI and its staff and resources can be found at the website: http://wucci.wustl.edu

To qualify for a DRC-WUCCI Scholarship, you must be a current DRC member. DRC members are eligible for only one scholarship, and these are available on a first come, first served basis. To apply please contact the WUCCI Scientific Director, James Fitzpatrick, Ph.D. at fitzp@wustl.edu with your name, department / division and a short description (2-3 paragraphs) of your project and what WUCCI resources you plan to use. 

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Please acknowledge the Washington University DRC in your publications as follows: "Supported by DRC, Grant No. P30 DK020579"

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Current DRC Pilot and Feasibility Projects

Year

Investigator

Title

Present Position

2014

Brian Finck, PhD

Mitochondrial pyruvate transport and beta cell function

Assistant Professor, Nutritional Sciences

2014

Simon Haroutounian, PhD

Predicting individual response to analgesic treatment in painful diabetic neuropathy and improving nonverbal

assessment of pain relief

Assistant Professor, Anesthesiology

2014

Colin Nichols, PhD

Zebrafish model of excitability-driven diabetes

Professor, Cell Biology & Physiology

2014

M. Yanina Pepino de Gruev, PhD

Metabolic effects of non-nutritive sweeteners

Assistant Professor, Nutritional Sciences

2015

Alison G. Cahill, MD

Choline metabolomics in diabetic pregnancy

Associate Professor, Obstetrics and Gynecology

2015

Andrew R. Coggan, PhD

Dietary nitrate-a novel treatment for patients with diabetes and heart failure

Assistant Professor, Radiology

2015

Jeffrey R. Millman, PhD

Improved differentiation of human stem cells to beta cells with oxygen control

Assistant Professor, Endocrinology, Metabolism & Lipid Research

2015

Babak Razani, MD, PhD

Use of a novel sugar to treat obesity and diabetes

Assistant Professor, Cardiology

2015

Dmitri Samovski, PhD

Regulation of insulin and AMPK signaling by saturated fatty acids

Instructor, Nutritional Sciences

2015

Mohamed Zayed, MD, PhD

N-Acetyl-Cysteine for healing of amputation stumps in the setting of diabetes

Assistant Professor, Surgery

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Shared National Resource Consortium

University of Kentucky Current DRC Pilot and Feasibility Projects

Year

Investigator

Title

Present Position

2015

Ming C. Gong, MD, PhD

Eating at the right time - A novel approach to correct non-dipping blood pressure

Professor, Physiology, University of Kentucky

2015

Ann C. Morris, PhD

The effects of hyperglycemia on visual system development

Assistant Professor, Biology,  University of Kentucky

2015

Bradley K. Taylor, PhD

Mechanisms and treatment of type II painful diabetic neuropathy

Professor, Physiology, University of Kentucky

University of Utah Current DRC Pilot and Feasibility Projects

Year

Investigator

Title

Present Position

2015

John D. Symons, PhD

Pathophysiological and genetic disruption of EC autophagy lowers EC NO production

Professor, Nutrition & Integrated Physiology, University of Utah

2015

Junko S. Warren, PhD

Role of abnormal Na/K pump in mitochondrial energetics in diabetic cardiomyopathy

Assistant Professor, Cardiovascular Research & Training Institute, University of Utah